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Sensitive 10 Minute Antibody Test for SARS-CoV-2 Developed

April 30th, 2020 Siavash Parkhideh Medicine, Public Health

A team of researchers at the Southern Medical University, Guangzhou, China, and collaborators report development of a rapid diagnostic assay for detection of SARS-CoV-2 antibodies in human blood. They report accurate detection with patient samples, with only 10 minutes to get a readout per blood sample.

The new method is a lateral flow assay, much like a home pregnancy test. Human serum is obtained from a test patient and added onto one side of the device, where it flows through the paper due to capillary action. The researchers impregnated COVID-19 proteins in a thin line on this paper to bind the antibodies. If the anti-COVID-19 antibodies are present, they will bind along this line, else, there will be no binding activity. To improve resolution, the researchers also use secondary binding of rabbit anti-human IgG antibodies, linked with a fluorescent reporter, allowing for greater accuracy than an unassisted visual readout.  

The researchers tested the assay on seven serum samples from known COVID-19 positive patients, and twelve patients indicated as negatives by RT-PCR. The new assay correctly diagnosed all seven samples as positive, and a negative case that had suspicious clinical symptoms was also found to contain COVID-19 human antibodies. The test took only 10 minutes per patient and the researchers say the test could help confirm negative diagnosis, monitor COVID-19 recovery, study past exposure, and identify recovered individuals with high antibody levels as plasma donors.

The publication in journal Analytical Chemistry: Rapid and sensitive detection of anti-SARS-CoV-2 IgG using lanthanide-doped nanoparticles-based lateral flow immunoassay

Via: American Chemical Society

Siavash Parkhideh

A PhD student at Rice University, Siavash Parkhideh is a researcher passionate about biomaterials, diabetes, and cell therapy. He hopes to advance the field, and leverage science to create impactful products and technology that improve the lives of others. Siavash has worked on projects related to cell engineering and implantable cell therapies. When he’s not pipetting, you may find him reading Hemingway or biking.

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