Yale University researchers have developed a new microscopy technique that allows them to have a 3D view of the insides of entire cells.
Dubbed whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), the method improves on a technique called 4Pi that uses two objectives to provide a 3D version of super-resolution microscopy. It allows researchers to pick specific structures to look at and the team that developed the technology has already visualized the endoplasmic reticulum, bacteriophages, mitochondria, nuclear pore complexes, primary cilia, Golgi-apparatus-associated COPI vesicles, and mouse spermatocyte synaptonemal complexes.
Here’s a video produced by the microscopy technique of 19 mouse chromosomes:
Study in journal Cell: Ultra-High Resolution 3D Imaging of Whole Cells…