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New Technique Enables High-Throughput, Lower Cost Imaging of Viral Nano-Particles

March 6th, 2013 Gavin Corley Diagnostics, Nanomedicine, News, Public Health

imaging-of-viral-nanoparticlesA team of scientists from University of California Los Angeles and MINATEC innovation campus in Grenoble, France have demonstrated a new imaging technique which could enable larger throughput, lower cost detection of viruses in resource-limited point of care settings.

Particles with a size measured in nanometers are generally too small to be observed using regular optical microscopy. While existing nano-particle imaging systems such as electron microscopy can provide excellent imaging resolution, they are generally limited by high cost, low-throughput and a limited field of view. The research team overcame these limitations by introducing an additional sample preparation step in which liquid nano-lenses were assembled around nano-particles in the sample.

The prepared sample is imaged using digital in-line holography during which the nano-lenses create diffraction patterns caused by their interaction with the holographic light source. These patterns are then detected by a CMOS imaging sensor, reconstructed and analysed for the presence of nano-particles.

While this general approach has been used previously, the presence of the self assembled nano-lenses enabled the researchers to achieve a greater imaging resolution in the sub-micrometer range over the entire CMOS sensor area (~20.5 mm sq.). The researchers believe this will have immediate applications for high throughout point of care detection of viral nano-particles.

To date the team has demonstrated the proof of concept of the technique by imaging sub-100 nanometer adenoviruses and single H1N1 viral particles, and their findings have been published in the journal Nature Photonics.

Study abstract: Wide-field optical detection of nanoparticles using on-chip microscopy and self-assembled nanolenses

 

Gavin Corley

Gavin holds a first-class Bachelor’s degree in Electronic Engineering degree from the University of Limerick and a Ph.D. in Biomedical Engineering from the National University of Ireland, Galway. He is currently a post-doctoral research and project leader at the NUI Galway where he has spent the last 2 years leading the product development and commercialization of two novel technologies for the management of varicose veins and chronic venous leg ulcers. He has published several research papers in the fields of anatomy, physiology and medical physics and is the lead author of one patent application to date. In his free time Gavin has been known to swim, cycle and hum a tune or two.

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