Transmission electron microscopy relies on electronic lenses that focus the signal much like their optical brothers. And like in optical microscopy, the lenses themselves present challenges and barriers to achieving higher resolution imaging.
Now researchers at University of Sheffield have demonstrated a new method, called electron ptychography, that does away with the lenses and uses computers to reconstruct the unfocused signal. The team believes the technology’s resolution could be pushed much higher in the future because the method doesn’t suffer from experimental boundaries created by the limits of the laws of physics.
Project leader Professor John Rodenburg, of the University of Sheffield´s Department of Electronic and Electrical Engineering, said: “To understand how material behaves, we need to know exactly where the atoms are. This approach will enable us to look at how atoms sit next to one another in a solid object as if we´re holding them in our hands.
“We´ve shown we can improve upon the resolution limit of an electron lens by a factor of five. An extension of the same method should reach the highest resolution transmission image ever obtained; about one tenth of an atomic diameter. No longer does TEM have to be bound by the paradigm of the lens, its Achilles´ heel since its invention in 1933.”
The technique is applicable to microscopes using any type of wave and has other key advantages over conventional methods. For example, when used with visible light, the new technology forms a type of image that means scientists can see living cells very clearly without the need to stain them, a process which usually kills the cells.
The new method also disposes of the need to put a lens very close to a living sample, meaning that cells can be seen through thick containers like petri dishes or flasks. This means that as they develop and grow over days or weeks, they do not have to be disturbed.
Plans are even being put into place with the European Space Agency to take the new, more robust, microscope technology to the moon in 2018 to examine the structure of moon soil.
Professor Rodenburg added: “We measure diffraction patterns rather than images. What we record is equivalent to the strength of the electron, X-ray or light waves which have been scattered by the object – this is called their intensity. However, to make an image, we need to know when the peaks and troughs of the waves arrive at the detector – this is called their phase.
“The key breakthrough has been to develop a way to calculate the phase of the waves from their intensity alone. Once we have this, we can work out backwards what the waves were scattered from: that is, we can form an aberration-free image of the object, which is much better than can be achieved with a normal lens.
“A typical electron or X-ray microscope image is about one hundred times more blurred than the theoretical limit defined by the wavelength. In this project, the eventual aim is to get the best-ever pictures of individual atoms in any structure seen within a three-dimensional object.”
Press release: Scientists revolutionise electron microscope
Abstract in Nature Communications: Ptychographic electron microscopy using high-angle dark-field scattering for sub-nanometre resolution imaging
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