Researchers at Karlsruhe Institute of Technology (KIT) in German province Baden-Württemberg have developed a 3D two-component polymer extracellular matrix, that can specifically control parameters of attached cells, such as their shape and volume:
It was the objective of the group under the direction of Bastmeyer [Professor Martin Bastmeyer –ed.] to develop defined three-dimensional growth substrates for the cell culture. The cells are to adhere at certain points only rather than randomly. In this way, parameters, such as the cell shape, cell volume, intercellular force development, or cellular differentiation can be determined systematically as a function of the external geometry of the surroundings. These findings are needed for the later specific larger-scale production of three-dimensional growth environments for tissue cultures required in regenerative medicine, for instance.
This objective was reached by means of a special polymer scaffold. The scaffold consists of a flexible, protein-repellent polymer with small box-shaped holds made of a protein-binding material. For scaffold construction, the scientists used the Direct Laser Writing Method (DLS) developed by the physicists Professor Martin Wegener and Professor Georg von Freymann at CFN. By means of this process, the protein-repellent structure was fabricated. It consists of 25 µm high pillars that are connected by thin bars at various heights. In a second lithography step, the holds were placed exactly in the middle of the bars. With the help of a solution of adhesion proteins, the proteins only bind to these small holds. Within two hours, individual cells colonize the scaffolds and adhere to the given adhesion points only.
For the first time, the scientists of CFN, Karlsruhe, succeeded in producing suitable materials, in which the growth of individual cells can be controlled and manipulated specifically in three dimensions. This is an important step towards the general understanding of how the natural three-dimensional environment in the tissue influences the behavior of cells.
Full story: Third Dimension of Specific Cell Cultivation …
Abstract in Advanced Materials: Two-Component Polymer Scaffolds for Controlled Three-Dimensional Cell Culture