Cell Levitation to Build 3D Matrix Structures

Jokes about needing special glasses aside, Nature Nanotechnology published a letter on March 14, 2010 describing progress in three dimensional cell culture technology from Glauco Souza, et. al. at the Texas M.D. Anderson Cancer Center. According to the research team, prior attempts at 3-D culture have included “protein based gel environments or rotational/agitation-based bioreactors” and yet “broad, practical application of such methods has not been achieved.” The novel method they describe uses magnetic fields to manipulate cells which have endocytosed “gold-hydrogels” which incorporate magnetic iron oxide. Once the cells have taken up the iron in the hydrogel, a magnetic field is applied which levitates the cells, allowing them to grow in a three dimensional architecture as opposed to the standard two dimensional fashion.
One benefit of this technology as reported in the letter is the flexibility of the cell culture medium. Current products available use a fixed chemical environment in their scaffolding to support three dimensional growth of cells. Because certain cell populations have specific metabolic requirements which must be met by the culture medium, the fixed chemical environment of existing 3-D culture techniques may preclude specific cell populations from being used. However, because this technology does not rely on a chemical environment, cell lines are not limited by the medium they grow in but rather the ability to take in the iron laced hydrogel.
The researchers state the potential applications of their work include “biotechnology, drug discovery, stem cell research, or regenerative medicine.” They go on to say, “Indeed, a potential long-term goal is the possibility of accomplishing the ‘engineering’ of normal tissues or complex organs.” The technology has been licensed to n3D Biosciences out of Houston, Texas.
M. D. Anderson press release: 3-D Cell Culture: Making Cells Feel Right at Home
Abstract in Nature Nanotechnology: Three-dimensional tissue culture based on magnetic cell levitation
Link: n3D Biosciences…