Scientists from the Max Planck Institute of Colloids and Interfaces have developed a new process to put together nanoparticles directly in the environment that is being studied. Additionally, this technique has led to the creation of tiny “light bulbs” that can be attached to specific proteins, opening a new modality for visualizing biochemical processes.
From a statement by the Max Planck Society:
“We used the fact that cells represent a closed reaction container as a model for the synthesis of nanoparticles,” says Rumiana Dimova. Her group at the Max Planck Institute of Colloids and Interfaces studies membranes – the cell envelope. The scientist and her colleagues form bubbles that are around 50 micrometres in size from lecithin membranes, which are similar to biological membranes. Like cells, membrane bubbles – or vesicles as scientists refer to them – also provide a closed reaction container. The scientists load the membrane bubbles with one of two reactants for the nanoparticles.
From this point, the researchers have developed two different sets of protocols. In one case, they produce bubbles loaded with one of the two reactants, sodium sulphide or cadmium chloride. The scientists then bring the bubbles with the different loads together and fuse two vesicles to form a bigger vesicle – this is done by subjecting the bubble cocktail to a short but very strong electrical pulse. The electric shock fuses the membranes of two adjacent bubbles.
In many cases, this results in the fusion of two bubbles containing different reactants. These then react to form cadmium sulphide, which is not water soluble and thus precipitates in the form of nanoparticles. “Because the reactants are only present to a limited extent in the fused bubbles, the particles only grow to a size of four nanometres,” explains Rumiana Dimova. The scientists were able to track the entire process directly under the microscope because they had added different fluorescent molecules to the membranes of the differently loaded vesicles. The researchers were also able to see the nanoparticles forming as the particles shone like tiny lamps.
In the second process, the researchers only produce vesicles with one of the reactants. When the vesicles have formed, unlike in the first procedure, the researchers do not remove them from the production chamber. Instead, the bubbles remain attached to their substrate via small membrane channels, like balloons tied to strings, and stand in a solution that is the same as the one inside them. The researchers working with Rumiana Dimova then altered this situation: they substituted the solution with the first ingredient for the nanoparticles with a second component. This causes no change inside the vesicles at first. The second ingredient only creeps gradually between the substrate and membrane into the channel and to the vesicle. In the vesicle, where the other ingredient is already waiting, the nanoparticles grow again – this time to a size of 50 nanometres.
Press release: Making nanoparticles in artificial cells…
