MIT researchers have developed a method to restrain live tiny worms, a key step allowing for high precision experimental surgery on nerves to study neuro regeneration.
Led by Mehmet Fatih Yanik, MIT assistant professor of electrical engineering and computer science, the team reported its latest work in the April 2 advanced online issue of the journal Lab on a Chip. The work involves the C. elegans worm, one of the tiniest multi-cellular organisms known. Smaller than a human hair, the worm is considered a key model for investigating a variety of biological phenomena such as aging, fat metabolism and neurological diseases.
Geneticists have been studying C. elegans since the 1960s, but the manual processes they used to do so were painstaking and time-consuming. That changed in a big way last year when Yanik and colleagues reported in the Proceedings of the National Academy of Science that they had developed a microfluidic chip to automate and accelerate research on the tiny worms. Essentially, the tiny worms are flowed inside the chip, immobilized by suction and imaged with a high-resolution microscope.
The research published this month goes one step further. Yanik and two collaborators, lead authors Fei Zeng, a postdoctoral fellow in the Research Laboratory of Electronics, and Christopher B. Rohde, a graduate student in electrical engineering and computer science, said they were able to render the animals motionless in the chip with an unprecedented stability for several minutes instead of seconds. This then allowed them not only to conduct three-dimensional imaging of the worms at the sub-cellular resolution but also to reliably operate on the animals with a high-precision surgery laser to study neural degeneration and regeneration on the chip. Yanik’s team had previously demonstrated that neural regeneration can be studied in C. elegans using femtosecond laser micro-surgery.
Press release: Live-animal nerve regeneration study gets a boost
Image caption: Figure illustrates “lab on a chip” technique developed by MIT researchers to allow immobilization and imaging of a live C. elegans worm: (a-i) worm moves freely in chip’s microfluidic channel; (a-ii) partial immobilization of worm; (a-iii) full immobilization; (b) low magnification image of worm immobilized in the device; (c) close-up of immobilized worm showing animal’s neurons (fluorescent). Image courtesy / Mehmet Fatih Yanik”>Figure illustrates “lab on a chip” technique developed by MIT researchers to allow immobilization and imaging of a live C. elegans worm: (a-i) worm moves freely in chip’s microfluidic channel; (a-ii) partial immobilization of worm; (a-iii) full immobilization; (b) low magnification image of worm immobilized in the device; (c) close-up of immobilized worm showing animal’s neurons (fluorescent). Image courtesy / Mehmet Fatih Yanik…
