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Nanopore-based Single-Molecule Spectroscopy

September 5th, 2007 Medgadget Editors Nanomedicine


Michael Berger at the Nanowerk blog has an article on the latest efforts to use nanopores to characterize proteins and sequence single DNA molecules. His report focuses on successful experiments that demonstrated the feasibility of single-molecule DNA through-the-pore spectroscopy. On our pages, we wrote on a number of promising experiments in which scientists developed special nanopore channels, that interacted with translocating molecules and “reported” their molecular structure and sequence (see flashbacks below).
From the current Nanowerk article:

“In recent years, the creation of nanochannels or nanopores in thin membranes has attracted much interest due to the potential to isolate and sense single DNA molecules while they translocate through the highly confined channels” Dr. Joshua Edel, a lecturer in micro- and nanotechnology at the Imperial College London, explains to Nanowerk. “Nanopores for such applications have already been fabricated but in all studies to date, the detection of translocation events is performed electrically by measuring the ionic current” (what this means is that molecules translocating through a nanopore will momentarily perturb the ionic current, with the duration of the perturbation and the magnitude of the current blockade providing more detailed information about molecular shape and structure).
Edel’s group, together with collaborators from Drexel University, recently presented proof-of-concept studies that describe a novel approach for optically detecting DNA translocation events through an array of solid-state nanopores which allows for ultrahigh-throughput, parallel detection at the single-molecule level (“Single-Molecule Spectroscopy Using Nanoporous Membranes”).

More from Nanowerk…
Flashbacks: Using Nanopore Channels to Sort DNA; Nanopore Method for DNA Sequencing

Medgadget Editors

Medical technologies transform the world! Join us and see the progress in real time. At Medgadget, we report the latest technology news, interview leaders in the field, and file dispatches from medical events around the world since 2004.

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