Cornell investigators are reporting a discovery explaining one of the most fundamental aspects of the DNA replication process. With the help of a micron-sized plastic bead attached to the DNA, Michelle Wang and colleagues from the Dept. of Physics, have shown that DNA double helix is actively separated by a helicase enzyme. The group’s research was published in the latest issue of Cell:
Scientists have known that helicases bind to the area of a double helix where the two strands fork away from each other, like the free ends of two pieces of thread wound around each other. The forked area opens and closes very rapidly. But scientists have debated whether helicases actively separate the two strands at the fork or if they passively wait for the fork to widen on its own.
The research found that the helicase appears to actively exert a force onto the fork and separate the two strands.
“A simple passive unwinding mechanism does not explain our data,” said Michelle Wang, associate professor of physics and the paper’s senior author.
“Defects in helicases are associated with many human diseases, ranging from predisposition to cancer to premature aging,” said co-author Smita Patel, a biochemistry professor at the Robert Wood Johnson Medical School in Piscataway, N.J. “Helicases are involved in practically all DNA and RNA metabolic processes.”
The researchers made their discovery by anchoring one end of one of the strands in a double helix to the surface of a microscope cover slip. The end of the other strand was attached to a micron-sized plastic bead. They then focused a laser beam on the tiny bead and trapped the bead in place within the beam of light. This setup allowed the researchers to measure the position and force on the bead, creating a very precise sensor of the helicase motion. As the helicase moved toward the fork and the double helix unwound, the tension on the two strands lessened. Using statistical mechanics models, the researchers could then compare actual measurements of movement with predictions based on both active and passive scenarios.
“The unwinding has to have some active component to it, and based on our data, we can tell you exactly how active it is,” said Wang. “Basically, it is an active unwinding motor.”
While helicases unwind very rapidly in cells, in test tube experiments the unwinding is much slower. The researchers believe that helicases work with other enzymes, where “accessory proteins are helping the helicase out by destabilizing the fork junction,” said Wang.