Binding nanoparticles to proteins has been an especially difficult task. Thanks to new research reported by investigators from the Brookhaven National Laboratory, and published at Angewandte Chemie, the novel nanoparticle-protein complexes open a host of possibilities for the future of diagnostics, biomed research, and clinical applications:
“Our study demonstrates that nanoparticles are appealing templates for assembling functional biomolecules with extensive potential impact across the fields of energy conversion, structural biology, drug delivery, and medical imaging,” said lead author Minghui Hu, a postdoctoral student working with James Hainfeld, Raymond Brinas, Luping Qian, and Elena Lymar in the Biology Department at Brookhaven Lab…
For this research, the scientists attached gold nanoparticles to an enzyme complex that helps drug-resistant tuberculosis bacteria survive, which has been studied by Brookhaven Lab biologist Huilin Li. The researchers suggest that gold nanoparticles might also be tailored to inactivate this enzyme complex, thereby thwarting drug-resistant TB – a research avenue they may explore in future studies.
In another part of the study, the researchers used proteins found on the surface of adenovirus, a virus that causes the common cold. Previous studies by Broookhaven’s Paul Freimuth have characterized how this virus binds to the human cells it infects, and have suggested that modified forms of adenovirus could be used as vehicles to deliver drugs to specific target cells, such as those that make up tumors.
One key to this approach would be to enhance strong binding to the target cells. Toward that end, Hu and Hainfeld’s group attached multiple viral proteins to the gold nanoparticles. Such constructs should have increased binding affinity for target cells and their larger size should extend blood residence time for improved drug delivery.
In another application, this new research showed that gold nanoparticles can enhance scientists’ ability to decipher the structures and functionally important regions of protein molecules – the workhorses that carry out every function of living cells and whose dysfunction often leads to disease. With added nanoparticles, the “signal-to-noise ratio” and resolution of an imaging technique known as cryo-electron microscopy were significantly increased. This method might enable analysis of small biological macromolecules and complexes that are currently intractable to analyze by cryo-electron microscopy or x-ray crystallography.
Throughout this work, the biggest challenge was to synthesize size-controllable nanoparticles coated with organic molecules designed to react with specific protein sites. Hu explains the steps: “First, we design the specific interactions between gold nanoparticles and the proteins by coating the gold nanoparticles with functional organic molecules using a biocompatible linker. Then we add a genetically engineered sequence of peptides, called a “tag,” to the protein molecule, which acts as the binding site for the gold nanoparticles. Finally, we incubate the nanoparticles with the protein solution to allow the nanoparticles and proteins to bind, transfer the solution onto a transmission electron microscopy grid, and analyze the complexes using state-of-the-art electron microscopes.”
Press release: Pairing Nanoparticles with Proteins …
