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Observing Prions in Action

May 11th, 2007 Medgadget Editors News

Investigators under MIT biology professor Susan Lindquist are doing some remarkable experiments to investigate pathophysiology of prionic diseases:

Although prions have received a great deal of scrutiny, scientists still don’t understand many of the most fundamental mechanisms of how prions form, replicate and cross from one species to another.
Now, through studying nontoxic yeast prions, scientists at MIT and the Whitehead Institute have discovered small but critical regions within prions that determine much of their behavior.
“These findings provide a new framework for us to begin exploring properties of prion biology that, up until now, have proven difficult to investigate,” says Whitehead member and MIT biology professor Susan Lindquist, senior author of a paper that appeared in the May 9 online issue of Nature.
Proteins are the cell’s workhorses, and they need to fold into complex and precise shapes to do their jobs. Prions are proteins that start out normally, but then at some point misfold–rather like an origami swan that comes out looking and acting instead like a vulture.
But prions have another characteristic that enables them to wreak havoc. They recruit other, properly folded proteins into misforming along with them, a process Lindquist calls a “conformational cascade.” In many organisms, this conformational cascade creates long fibers called amyloids. (The brains of animals that have died from prion infections are literally packed with amyloid clumps.)
To glean insights into the mechanics that enable amyloid formation, Peter Tessier, a postdoctoral scientist in Lindquist’s lab, used peptide arrays–glass slides covered with thousands of tiny protein fragments. Traditionally, these arrays are used for finding binding sites within well-behaved proteins. Here, Tessier designed the arrays so that he could observe protein folding and amyloid formation in real time.
Tessier covered the array with peptides from baker’s yeast and then added prion protein to the array, also from the same yeast species. He found that a small cluster of peptides recruited the prion proteins to misfold into an amyloid structure. This region of the protein, which Tessier called a “recognition element,” constitutes about 10 percent of the prion. Tessier repeated this experiment with peptides and a prion taken from pathogenic fungi. The results were the same.
Both prions also maintained a rigid species barrier. The baker’s yeast prion could not recruit peptides from the pathogenic fungi cells, and vice versa.
To further verify these results, Tessier accessed a synthetic yeast prion, one that another research group had assembled from pieces of both the baker’s yeast and the pathogenic fungi prion. Earlier studies had shown that this synthetic prion could cross the species barrier but did not identify the mechanism. Tessier found that this synthetic prion contained two recognition elements, one for baker’s yeast and one for pathogenic fungi. When the prion was placed with peptide fragments from baker’s yeast, the baker’s yeast recognition element was activated, and likewise for the pathogenic fungi.
Even more striking, Tessier could activate different recognition elements by manipulating environmental conditions, such as temperature. For example, when he conducted the experiment at 4 degrees Celsius, the baker’s yeast recognition element switched on. At 37 degrees Celsius, the pathogenic fungi element was activated. In other words, temperature alone could dictate which yeast species the prion could infect. Additionally, the prion’s behavior could be altered by subtle alterations in the recognition element’s amino acid sequence.
While this prion is a laboratory construct not found in nature, these findings provide researchers with a new way to approach old questions, such as why some prion diseases can jump from one species to another but others can’t. Tessier and Lindquist say it is likely that natural prions contain more than one recognition element, and recognition elements can slide into a neighboring region. Many external factors can determine which recognition element is activated, in turn influencing the downstream behavior of the prion.
“These findings are remarkable for two reasons,” says Lindquist, who is also an investigator for Howard Hughes Medical Institute. “For one thing, this is the first time that these peptide arrays have been used to study protein folding. We’ve taken this platform to a whole new level. Also, we’ve seen just one small part of this prion inducing proteins to fold. This is an entirely new concept.”

MIT press release: Scientists ID secret to infectious protein …
MIT Tech Review: Prion Infectivity Pinpointed …

Medgadget Editors

Medical technologies transform the world! Join us and see the progress in real time. At Medgadget, we report the latest technology news, interview leaders in the field, and file dispatches from medical events around the world since 2004.

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