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Lasers Detect a Single Virus in 60 Seconds or Less

November 17th, 2006 Josh Umbehr Public Health

The University of Georgia’s physics professor Yiping Zhao and chemistry professor Richard Dluhy have, through trial and error, perfected the principles of the Raman Shift to rapidly detect viruses in biological samples.

Waiting a day or more to get lab results back from the doctor’s office soon could become a thing of a past. Using nanotechnology, a team of University of Georgia researchers has developed a diagnostic test that can detect viruses as diverse as influenza, HIV and RSV in 60 seconds or less.
In addition to saving time, the technique – which is detailed in the November issue of the journal Nano Letters – could save lives by rapidly detecting a naturally occurring disease outbreak or bioterrorism attack.
“It saves days to weeks,” said lead author Ralph Tripp, Georgia Research Alliance Eminent Scholar in Vaccine Development at the UGA College of Veterinary Medicine. “You could actually apply it to a person walking off a plane and know if they’re infected.”
The technique, called surface enhanced Raman spectroscopy (SERS), works by measuring the change in frequency of a near-infrared laser as it scatters off viral DNA or RNA. This change in frequency, named the Raman shift for the scientist who discovered it in 1928, is as distinct as a fingerprint.
This phenomenon is well known, but Tripp explained that previous attempts to use Raman spectroscopy to diagnose viruses failed because the signal produced is inherently weak.
But UGA physics professor Yiping Zhao and UGA chemistry professor Richard Dluhy experimented with several different metals and methods and found a way to significantly amplify the signal. Using a method they’ve patented, they place rows of silver nanorods 10,000 times finer than the width of a human hair on the glass slides that hold the sample. And, like someone positioning a TV antenna to get the best reception, they tried several angles until they found that the signal is best amplified when the nanorods are arranged at an 86-degree angle.
“The enhancement factors are extraordinary,” Dluhy said. “And the nice thing about this fabrication methodology is that it’s very easy to implement, it’s very cheap and it’s very reproducible.”
Tripp said the technique is so powerful that it has the potential to detect a single virus particle and can also discern virus subtypes and those with mutations such as gene insertions and deletions. This specificity makes it valuable as a diagnostic tool, but also as a means for epidemiologists to track where viruses originate from and how they change as they move through populations.

Read the full article . . .

Josh Umbehr

Josh Umbehr, MD founded AtlasMD Concierge Family Practice, one of the fastest growing insurance-free practices in the country. Currently developing an industry leading platform for direct primary care physicians: Atlas EMR Software for Concierge Medicine.

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