Investigators at the U.S. Department of Energy’s Brookhaven National Laboratory are describing a novel method to assemble gold nanoparticles, using DNA as a template:
“In biology, DNA is mainly an informational material, while in nanoscience, DNA is an excellent structural material due to its natural ability to self-assemble according to well-specified programmable rules,” said Oleg Gang, the Brookhaven physicist who leads the research team. “Using biological materials such as DNA, we are developing approaches to control the assembly of inorganic nano-objects. However, in order to really turn this attractive approach into nanotechnology, we have to understand the complexity of interaction in such hybrid systems.”
The synthetic DNA used in the laboratory is capped onto individual gold nanoparticles and customized to recognize and bind to complementary DNA located on other particles. This process forms clusters, or aggregates, of gold particles.
“It’s really by design,” Maye said. “We can sit down with a piece of paper, write out a DNA sequence, and control how these nanoparticles will assemble.” [Mathew Maye is a chemist at Brookhaven –ed.]
One limitation to the assembly process is the use of single-stranded DNA, which can bend backward and attach to the particle’s gold surface instead of binding with surrounding nanoparticles. This flexibility, along with the existence of multiple forms of single-stranded DNA, can greatly slow the assembly process. In the Brookhaven study, researchers introduced partially rigid, double-stranded DNA, which forces interacting linker segments of DNA to extend away from the gold surface, allowing for more efficient assembly.
“By using properties of DNA, we can increase assembly kinetics, or speed, by relatively simple means without a lot of synthetic steps,” Maye said.
The research team probed the synthesized and assembled nanosystems with multiple imaging techniques, using beams of light and electrons as well as high-intensity x-rays at Brookhaven’s National Synchrotron Light Source. The scientists look to further improve the controllability of the system, focusing next on the size of the nanoparticle clusters.