Cell-Death Protein and Its Role in Neurodegeneration

Here’s some interesting research from investigators at UPenn School of Medicine that might have implications in diagnosis and treatment of neurodegenerative disorders:

Researchers at the University of Pennsylvania School of Medicine discovered that a protein called Elk-1 interacts with mitochondria, the energy storehouse of a cell, suggesting that this protein – typically active in the nucleus – could play a role in cell death, and mitochondria-related diseases such as neurodegeneration and schizophrenia…
In the series of experiments (described in the PNAS study) to discern the nature of Elk-1’s role in the dendrite, the investigators first characterized some of the proteins with which Elk-1 interacts, and found that Elk-1 associates with mitochondria proteins. Mitochondria are distributed throughout cells, including in the dendrites, and are important in maintaining the energy stores and regulating viability and death of the cell.
The researchers then overexpressed Elk-1 in rat neurons to see if there was an effect on cell viability. “We thought that through interaction with Elk-1, the mitochondria would be able to regulate cell death,” says Eberwine. [James Eberwine, PhD, professor of pharmacology at UPenn -ed.] “By overexpressing Elk-1, we found that we did decrease cell viability, achieving more cell death. Conversely, when we knock-down Elk-1 expression, the survivability of neurons increased, which indicates that Elk-1 plays a role in neuron viability.”
Cell-death is a component of a number of psychiatric and neurological diseases such as schizophrenia and those that involve neurodegeneration. For many of these diseases dysfunction of the dendrite is also associated with the disease process. “Therefore, anything that impacts dendrite function might be associated with illness,” surmises Eberwine. “The fact that Elk-1 RNA and protein are present in dendrites, and the fact that Elk-1 can modulate cell viability, potentially through the mitochondria, suggests that Elk-1 could play a role in these diseases perhaps through modulation of mitochondrial activity.”
To more precisely understand the role of Elk-1 RNA in the dendrite, the researchers developed a method called phototransfection, which was described in their June Nature Methods paper, to focally introduce Elk-1 RNA into the dendrite. In this technique, a laser light beam is used to create small transient pores in the membrane of intact rat nerve cells, into which a known amount of RNA molecules are introduced by diffusion.
The introduction and translation of Elk-1 mRNA in dendrites by phototransfection also elicited cell death whereas introduction and translation of Elk-1 mRNA in the cell soma did not produce cell death. The Elk-1 proteins translated in the dendrites were transported to the nucleus and cell death depended on subsequent transcription. These results compliment and expand upon the PNAS study in which Elk-1’s involvement in cell death and association with mitochondria were elucidated.
“This is the first formal proof that RNA can be translated and made into protein in an intact neuronal dendrite,” explains Eberwine. “We have seen this with isolated dendrites before, but not in an intact cell.”
In the mouse model, Elk-1 mRNA is being made into protein on ribosomes located at the periphery of the nerve cell in the dendrite. Protein is also being made in the cell body, but there may be a difference in the Elk-1 protein made in the cell body. “We speculate that the dendrite’s environment comes into play, with kinases and phosphatases that modify the Elk-1 proteins made in the dendrite,” explains Eberwine. “We suggest that there’s a different phosphorylation signal on the protein made in the dendrite versus in the cell body.
“These studies highlight the importance of the dendritic environment in modifying proteins after they have been made,” concludes Eberwine. “There is a clear link between the nucleus and mitochondria via Elk-1, and it’s rapid. We don’t know exactly what that is, but it’s a very interesting signal in terms of neurodegeneration. These data provide intriguing new avenues for research, including determining the role of localized protein synthesis and protein modifications in dendrite-related pathologies, including Fragile X disease, schizophrenia, and autism. It is likely that not only are particular proteins going to be important in these diseases, which has been proven by genetics, but also the environment in which they are synthesized.”

The press release…